제품 > 항체 > 다중클론항체(pAb)

OTULIN Rabbit pAb (A20489)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OTULIN Rabbit pAb (A20489)

Western blot analysis of extracts of Rat liver, using OTULIN antibody (A20489) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - OTULIN Rabbit pAb (A20489)

Western blot analysis of extracts of MCF7 cells, using OTULIN antibody (A20489) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - OTULIN Rabbit pAb (A20489)

Immunofluorescence analysis of NIH/3T3 cells using OTULIN Rabbit pAb (A20489) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameOTULIN Rabbit pAb
Catalog No.A20489
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1(Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-352 of human OTULIN (NP_612357.4).
SequenceEHEEDMYRAADEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEARQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNHLNQVGHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIAEDDRHYNIPVRVCEETSL
Gene ID
Swiss Prot
SynonymsGUM; AIPDS; IMD107; FAM105B
Calculated MW40kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, Rat liver
Cellular locationcytoplasm, cytosol

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - OTULIN Rabbit pAb (A20489)}

    Western blot - OTULIN Rabbit pAb (A20489)

    Western blot analysis of extracts of Rat liver, using OTULIN antibody (A20489) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - OTULIN Rabbit pAb (A20489)}

    Western blot - OTULIN Rabbit pAb (A20489)

    Western blot analysis of extracts of MCF7 cells, using OTULIN antibody (A20489) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - OTULIN Rabbit pAb (A20489)}

    Immunofluorescence - OTULIN Rabbit pAb (A20489)

    Immunofluorescence analysis of NIH/3T3 cells using OTULIN Rabbit pAb (A20489) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)