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OTX2 Rabbit pAb (A14036)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunohistochemistry - OTX2 Rabbit pAb (A14036)

Immunohistochemistry analysis of paraffin-embedded rat brain using OTX2 Rabbit pAb (A14036) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - OTX2 Rabbit pAb (A14036)

Immunohistochemistry analysis of paraffin-embedded mouse brain using OTX2 Rabbit pAb (A14036) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - OTX2 Rabbit pAb (A14036)

Immunofluorescence analysis of mouse brain cells using OTX2 Rabbit pAb (A14036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameOTX2 Rabbit pAb
Catalog No.A14036
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human OTX2 (NP_068374.1).
SequenceTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNA
Gene ID
Swiss Prot
SynonymsCPHD6; MCOPS5; OTX2
Calculated MW32kDa
Observed MWRefer to figures
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationNucleus

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ABclonal:Immunohistochemistry - OTX2 Rabbit pAb (A14036)}

Immunohistochemistry - OTX2 Rabbit pAb (A14036)

Immunohistochemistry analysis of paraffin-embedded rat brain using OTX2 Rabbit pAb (A14036) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - OTX2 Rabbit pAb (A14036)}

Immunohistochemistry - OTX2 Rabbit pAb (A14036)

Immunohistochemistry analysis of paraffin-embedded mouse brain using OTX2 Rabbit pAb (A14036) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - OTX2 Rabbit pAb (A14036)}

Immunofluorescence - OTX2 Rabbit pAb (A14036)

Immunofluorescence analysis of mouse brain cells using OTX2 Rabbit pAb (A14036) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)