제품 > 항체 > 다중클론항체(pAb)

OsARF19 Rabbit pAb (A20587)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsARF19 Rabbit pAb (A20587)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsARF19 Rabbit pAb (A20587) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 600s.

Overview

Product nameOsARF19 Rabbit pAb
Catalog No.A20587
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 800-900 of Oryza sativa OsARF19 (Q0D9R7).
SequenceQPILGRNKHCSMINTEKVPQSAAPMSVPSSLEAVTATPRMMKDSPKLNHNVKQSVVASKLANAGTGSQNYVNNPPPTDYLETASSATSVWLSQNDGLLHQN
Gene ID
Swiss Prot
SynonymsARF19; ARF7A; OsARF7a; OJ1215_E11.1; OsARF19
Calculated MW128kDa
Observed MW110kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSpikes
Cellular locationnucleus
    ABclonal:Western blot - OsARF19 Rabbit pAb (A20587)}

    Western blot - OsARF19 Rabbit pAb (A20587)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsARF19 Rabbit pAb (A20587) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 600s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)