제품 > 항체 > 다중클론항체(pAb)

OsDLT Rabbit pAb (A20318)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsDLT Rabbit pAb (A20318) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product name	OsDLT Rabbit pAb
Catalog No.	A20318
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Probable transcription factor that acts as positivie regulator of brassinosteroid (BR) signaling. Functions downstream of BRI1 and GSK2 to modulate BR responses. Acts as a direct target of GSK2 kinase to mediate BR responses. Involved in feedback inhibition of BR biosynthetic genes. Repressed by BZR1. Cooperatively functions in a transactivating complex with SMOS1 to enhance the transcription of the SMOS1 target PHI-1, and regulate plant organ size. Interaction between SMOS1 and DLT is a crosstalk point for auxin and brassinosteroid signaling

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-214 of Oryza sativa OsDLT. (Q9LWU9).
Sequence	MLAGCSFSSSRHQMSTAQRFDILPCGFSKRGSRGDGAAPRVAGDARSGATTCSFRTHPAPPVTQSVSWGAKPEPGGNGNGAHRAVKRAHDEDAVEEYGPIVRAKRTRMGGDGDEVWFHQSIAGTMQATAAGEGEEAEEEKVFLVPSAAAFPHGMAAAGPSLAAAKKEEYSKSPSDSSSSSGTDGGSSAMMPPPQPPEFDARNGVPAPGQAEREA
Gene ID
Swiss Prot
Synonyms	DLT; OsGRAS-32; D62; GS6; SMOS2; OsDLT
Calculated MW	66kDa
Observed MW	66kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	young leaves (10 d), stems (before flower), leaves sheath (before flower)
Cellular location	nucleus.