제품 > 항체 > 다중클론항체(pAb)

OsEIL1 Rabbit pAb (A18629)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsEIL1 Rabbit pAb (A18629)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsEIL1 Rabbit pAb (A18629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameOsEIL1 Rabbit pAb
Catalog No.A18629
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transcription factor acting as a positive regulator in the ethylene response pathway). Required for the inhibition of root growth by ethylene in etiolated seedlings. Functions upstream of the auxin biosynthetic gene YUCCA8 and directly activates its expression . Functions downstream of the ethylene signaling factor EIN2 in disease resistance against the rice blast fungus (Magnaporthe oryzae. Binds directly to the promoters of the NADPH oxidases RBOHA and RBOHB, and the jasmonate biosynthetic gene OPR4 to activate their expression during fungal infection. May enhance disease resistance by facilitating reactive oxygen species (ROS) generation and jasmonate biosynthesis with subsequent phytoalexin accumulation during Magnaporthe oryzae infection. Acts as negative regulator of salt tolerance . During salt stress, activates the cation transporter HKT1, which mediates increased sodium uptake in roots, and contributes to sodium accumulation and salt toxicity. Binds directly to the DNA sequence 5'-TGTTACAAATACC-3' in the promoter of the GA20OX2 gene to activate its expression at the transcriptional level during ethylene signaling. Possesses transactivation activity in protoplasts.
ImmunogenLSRGKDPAGGVVGDPSK-C
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsEIL1; MHZ6; EIL1A; OsEIL1; OsEIL1a; OsJ_10672
Calculated MW71kDa
Observed MW90kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesLeaves
Cellular locationnucleus
    ABclonal:Western blot - OsEIL1 Rabbit pAb (A18629)}

    Western blot - OsEIL1 Rabbit pAb (A18629)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsEIL1 Rabbit pAb (A18629) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)