제품 > 항체 > 다중클론항체(pAb)

OsEIN2 Rabbit pAb (A20749)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsEIN2 Rabbit pAb (A20749)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsEIN2 Rabbit pAb (A20749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameOsEIN2 Rabbit pAb
Catalog No.A20749
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Central factor in ethylene signaling pathways that control development, senescence and grain size. Acts as positive component of the ethylene-signaling pathway.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 576-710 of Oryza sativa OsEIN2 (Q0D8I9).
SequenceVDWPEPLEPISNAIVAEESTVESVDSKSTGERDIEVEPALLMDNDKEAPNILESDNKPLGGNNPSCASDDGPPSLTFSRGKGSDAGNGSGSLSRLSGLGRAARRQLAAILDEFWGHLFDYHGKLTQEASSKRFDI
Gene ID
Swiss Prot
SynonymsEIN2; MHZ7; OsEIN2; P0455F03.4
Calculated MW138kDa
Observed MW138kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems, spikes
Cellular location
    ABclonal:Western blot - OsEIN2 Rabbit pAb (A20749)}

    Western blot - OsEIN2 Rabbit pAb (A20749)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsEIN2 Rabbit pAb (A20749) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)