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OsGBSSⅠ Rabbit pAb (A19151)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsGBSSⅠ Rabbit pAb (A19151)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGBSSⅠ Rabbit pAb (A19151) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameOsGBSSⅠ Rabbit pAb
Catalog No.A19151
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Required for the synthesis of amylose in endosperm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of Oryza sativa OsGBSSⅠ. (Q0DEV5).
SequenceMSALTTSQLATSATGFGIADRSAPSSLLRHGFQGLKPRSPAGGDATSLSVTTSARATPKQQRSVQRGSRRFPSVVVYATGAGMNVVFVGAEMAPWSKTGG
Gene ID
Swiss Prot
SynonymsWx; WX1; Wx-b; waxy; GBSS1; WX-mq; WX1-1; Wx-oz; GBSS-I; OsGBSS1; 134P10.7; OsGBSSⅠ
Calculated MW66kDa
Observed MW66kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesflower buds (before flower), flag leaves (after flower), flowers, milk seeds
Cellular locationamyloplast, chloroplast
Customer validation

WB(Golden Promise, Oryza sativa)

    ABclonal:Western blot - OsGBSSⅠ Rabbit pAb (A19151)}

    Western blot - OsGBSSⅠ Rabbit pAb (A19151)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGBSSⅠ Rabbit pAb (A19151) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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