제품 > 항체 > 다중클론항체(pAb)

OsMADS1 Rabbit pAb (A20328)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsMADS1 Rabbit pAb (A20328)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsMADS1 Rabbit pAb (A20328) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product nameOsMADS1 Rabbit pAb
Catalog No.A20328
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Probable transcription factor involved in the development of floral organs. Required for the formation of inner floral organs (lodicules, stamens and carpels, or whorls 2, 3 and 4 and the lemma and palea (whorl 1, which are grass floral organs analogous to sepals. May be involved in the control of flowering time. Seems to act as transcriptional activator. May act upstream of the auxin-responsive protein GH3.8.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 150-257 of Oryza sativa OsMADS1. (Q10PZ9).
SequenceDLKSKEQQLQDLNKDLRKKLQETSAENVLHMSWQDGGGHSGSSTVLADQPHHHQGLLHPHPDQGDHSLQIGYHHPHAHHHQAYMDHLSNEAADMVAHHPNEHIPSGWI
Gene ID
Swiss Prot
SynonymsLhs1; MADS1; OsMADS1; OsJ_09913
Calculated MW30kDa
Observed MWRefer to figures
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesFlower Buds, flowers, milk seeds
Cellular locationnucleus
Customer validation

WB(Oryza sativa)

ChIP(Oryza sativa)

RNA-Seq(Oryza sativa)

    ABclonal:Western blot - OsMADS1 Rabbit pAb (A20328)}

    Western blot - OsMADS1 Rabbit pAb (A20328)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsMADS1 Rabbit pAb (A20328) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20328? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)