제품 > 항체 > 다중클론항체(pAb)

OsMADS57 Rabbit pAb (A20658)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsMADS57 Rabbit pAb (A20658)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsMADS57 Rabbit pAb (A20658) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

Overview

Product nameOsMADS57 Rabbit pAb
Catalog No.A20658
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transcriptional factor that targets the CArG motif 5'-C(A/T)TTAAAAAG-3' in the promoter of D14. Directly suppresses D14 expression to control the outgrowth of axillary buds.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-200 of Oryza sativa OsFD2. (Q6Z6W2).
SequenceHNLQESHKQLMGEELSGLGVRDLQGLENRLEISLRNIRMRKDNLLKSEIEELHVKGSLIHQENIELSRSLNVMSQQKLELYNKLQACEQRGATDANESSST
Gene ID
Swiss Prot
SynonymsMADS57; OsMADS57; OsJ_08264
Calculated MW27kDa
Observed MW30kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesStems, Spikes
Cellular locationnucleus
    ABclonal:Western blot - OsMADS57 Rabbit pAb (A20658)}

    Western blot - OsMADS57 Rabbit pAb (A20658)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsMADS57 Rabbit pAb (A20658) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.