제품 > 항체 > 다중클론항체(pAb)

OsMYB30 Rabbit pAb (A20623)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsMYB30 Rabbit pAb (A20623)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsMYB30 Rabbit pAb (A20623) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 600s.

Overview

Product nameOsMYB30 Rabbit pAb
Catalog No.A20623
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Acts as negative regulator of cold tolerance. Negatively regulates beta-amylase genes at the transcriptional level in response to cold stress. Suppresses beta-amylase gene expression by interacting with TIFY11A/JAZ9. Maltose produced by beta-amylases has a role in protecting cell membranes under cold stress conditions in rice and may contribute to the cold tolerance as a compatible solute.
ImmunogenRecombinant protein of Oryza sativa OsMYB30.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsOsMYB30
Calculated MW28kDa
Observed MW28kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSpikes
Cellular locationnucleus
    ABclonal:Western blot - OsMYB30 Rabbit pAb (A20623)}

    Western blot - OsMYB30 Rabbit pAb (A20623)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsMYB30 Rabbit pAb (A20623) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 600s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)