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OsNPR1 Rabbit pAb (A20582)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsNPR1 Rabbit pAb (A20582)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsNPR1 Rabbit pAb (A20582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameOsNPR1 Rabbit pAb
Catalog No.A20582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Key positive factor of disease resistance. Plays an essential role in benzothiadiazole (BTH)-induced resistance to the blast fungus disease caused by Magnaporthe oryzae. Involved in defense response against the bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Over-expression of NPR1/NH1 confers disease resistance to Xoo, but also enhances herbivore susceptibility . Functions as a transcriptional coactivator of TGA2.1 and LG2 in vitro. Involved in defense response against herbivore. Plants silencing NPR1/NH1 have increased herbivore-induced trypsin proteinase inhibitors and volatiles, which reduces the performance of the striped stem borer (SSB) Chilo suppressalis
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 475-582 of Oryza sativa OsNPR1. (Q9FDY4).
SequenceRQRTTVDLNESPFIMKEEHLARMTALSKTVELGKRFFPRCSNVLDKIMDDETDPVSLGRDTSAEKRKRFHDLQDVLQKAFHEDKEENDRSGLSSSSSSTSIGAIRPRR
Gene ID
Swiss Prot
SynonymsNH1; PR2; NPR1; NPR2; OsNH1; OsNPR1
Calculated MW64kDa
Observed MW72kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesspikes, milk grains
Cellular locationcytoplasm, nucleus
Customer validation

WB(Oryza sativa, Other)

    ABclonal:Western blot - OsNPR1 Rabbit pAb (A20582)}

    Western blot - OsNPR1 Rabbit pAb (A20582)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsNPR1 Rabbit pAb (A20582) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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