제품 > 항체 > 다중클론항체(pAb)

OsSERK2 Rabbit pAb (A20637)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsSERK2 Rabbit pAb (A20637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

Overview

Product name	OsSERK2 Rabbit pAb
Catalog No.	A20637
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

LRR receptor kinase involved in positive regulation of somatic embryogenesis and defense response against the rice blast fungus pathogen Magnaporthe oryzae. Involved in the positive regulation of receptor kinase-mediated immunity. Required for immunity mediated by the LRR receptor kinases XA21 and XA26/XA3 which recognize effectors from the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Required for the immune response mediated by the LRR receptor kinase FLS2 which recognizes specifically the bacterial flagellin (flg22) effector. Kinase activity and direct interaction with the immune receptors is critical for their function

Immunogen information

Immunogen	Recombinant protein of Oryza sativa OsSERK2.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	OsSERK1; OsSERK2
Calculated MW	70kDa
Observed MW	70kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	leaves
Cellular location	plasma membrane