제품 > 항체 > 다중클론항체(pAb)

OsTB1 Rabbit pAb (A20349)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsTB1 Rabbit pAb (A20349)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsTB1 Rabbit pAb (A20349) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameOsTB1 Rabbit pAb
Catalog No.A20349
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Probable transcription factor that functions as a negative regulator of lateral branching, presumably through its expression in axillary buds. Involved in the fine tuning of shoot branching. May function as an integrator of multiple signaling pathways to regulate the development of axillary buds. Works partially downstream of strigolactones to inhibit bud outgrowth. Binds to MADS57 to suppress the negative regulation of D14 by MADS57 and balance the expression of D14 for tillering
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 270-388 of Oryza sativa OsTB1. (Q8LN68).
SequenceMRWVTLASAISVEAATAAAAAGEDKSPTSPSNNLNHSSSTNLVSTELEDGSSSTRHNGVGVSGGRMQEISAASEASDVIMAFANGGAYGDSGSYYLQQQHQQDQWELGGVVYANSRHYC
Gene ID
Swiss Prot
SynonymsTCP8; OsTB1
Calculated MW42kDa
Observed MW52kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems (before flower), leaves sheath (before flower), leaves (after flower)
Cellular locationnucleus
    ABclonal:Western blot - OsTB1 Rabbit pAb (A20349)}

    Western blot - OsTB1 Rabbit pAb (A20349)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsTB1 Rabbit pAb (A20349) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)