제품 > 항체 > 다중클론항체(pAb)

OsTE Rabbit pAb (A20368)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsTE  Rabbit pAb (A20368)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsTE Rabbit pAb (A20368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameOsTE Rabbit pAb
Catalog No.A20368
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of Oryza sativa OsTE. (Q10SH0).
SequenceMDHHHHHLPPPPPRSPMENSASSKPPTPASTPSSRLAAAPSSRVSSAAPHPSPSSSAPTPASRTVYSDRFIPSRAGSNLALFDLAPSPSHHDAAAAAASP
Gene ID
Swiss Prot
SynonymsTAD1; TE; OsCCS52A; OsTE
Calculated MW56kDa
Observed MW75kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves (before flower), leaves sheath (before flower)
Cellular location

Documents

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Lot number

    ABclonal:Western blot - OsTE  Rabbit pAb (A20368)}

    Western blot - OsTE Rabbit pAb (A20368)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsTE Rabbit pAb (A20368) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)