제품 > 항체 > 다중클론항체(pAb)

OsWRKY28 Rabbit pAb (A20572)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsWRKY28 Rabbit pAb (A20572)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsWRKY28 Rabbit pAb (A20572) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product nameOsWRKY28 Rabbit pAb
Catalog No.A20572
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is mainly expressed in roots before flowering (RPKM288.3) and leaves after flowering (RPKM21.0).Modulates plant innate immunity against X.oryzae pv. oryzae (Xoo) (PMID: 21961049).Regulates negatively the basal defense responses to the compatible fungus M.oryzae (PMID: 23462973, PMID: 21726398).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-240 of Oryza sativa OsWRKY28. (Q0DAJ3).
SequencePVLDFQYIQFMDSWIEQTSLSLDLNVGLPSTARRSSAPAAPIKVLVEENFLSFKKDHEVEALEAELRRASEENKKLTEMLRAVVAKYTELQGQVNDMMSAAAAAAVNAGNHQSSTSEGGSVSPSRKRIRSVDSLDDAAHHRKPSPPFVAAAAAAAYASPDQMECTSAAAAAAAKRVVREDCKPKVSKRFVH
Gene ID
Swiss Prot
SynonymsOsWRKY28
Calculated MW44kDa
Observed MW52kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves
Cellular location
    ABclonal:Western blot - OsWRKY28 Rabbit pAb (A20572)}

    Western blot - OsWRKY28 Rabbit pAb (A20572)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsWRKY28 Rabbit pAb (A20572) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)