제품 > 항체 > 다중클론항체(pAb)

OsWRKY53 Rabbit pAb (A20672)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsWRKY53 Rabbit pAb (A20672)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsWRKY53 Rabbit pAb (A20672) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameOsWRKY53 Rabbit pAb
Catalog No.A20672
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-181 of Oryza sativa OsWRKY53. (Q5W6M7).
SequenceMASSTGGLDHGFTFTPPPFITSFTELLSGGGGDLLGAGGEERSPRGFSRGGARVGGGVPKFKSAQPPSLPLSPPPVSPSSYFAIPPGLSPTELLDSPVLLSSSHILASPTTGAIPAQRYDWKASADLIASQQDDSRGDFSFHTNSDAMAAQPASFPSFKEQEQQVVESSKNGAAAASSNKS
Gene ID
Swiss Prot
SynonymsOsWRKY53
Calculated MW51kDa
Observed MW60kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems, Spikes
Cellular locationnucleus
    ABclonal:Western blot - OsWRKY53 Rabbit pAb (A20672)}

    Western blot - OsWRKY53 Rabbit pAb (A20672)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsWRKY53 Rabbit pAb (A20672) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.