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Osteoglycin (OGN) Rabbit pAb (A6679)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Osteoglycin (OGN) Rabbit pAb (A6679)

Western blot analysis of various lysates, using Osteoglycin (OGN) Rabbit pAb (A6679) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Osteoglycin (OGN) Rabbit pAb (A6679)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Osteoglycin (OGN) Rabbit pAb (A6679) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Osteoglycin (OGN) Rabbit pAb (A6679)

Immunohistochemistry analysis of paraffin-embedded Human stomach using Osteoglycin (OGN) Rabbit pAb (A6679) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Osteoglycin (OGN) Rabbit pAb (A6679)

Immunofluorescence analysis of U-2 OS cells using Osteoglycin (OGN) Rabbit pAb (A6679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameOsteoglycin (OGN) Rabbit pAb
Catalog No.A6679
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-298 of human Osteoglycin (OGN) (NP_054776.1).
SequenceAPPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF
Gene ID
Swiss Prot
SynonymsOG; OIF; SLRR3A; Osteoglycin (OGN)
Calculated MW34kDa
Observed MW35kDa/50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesC2C12, Mouse lung
Cellular locationSecreted, extracellular matrix, extracellular space

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ABclonal:Western blot - Osteoglycin (OGN) Rabbit pAb (A6679)}

Western blot - Osteoglycin (OGN) Rabbit pAb (A6679)

Western blot analysis of various lysates, using Osteoglycin (OGN) Rabbit pAb (A6679) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Osteoglycin (OGN) Rabbit pAb (A6679)}

Immunohistochemistry - Osteoglycin (OGN) Rabbit pAb (A6679)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Osteoglycin (OGN) Rabbit pAb (A6679) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Osteoglycin (OGN) Rabbit pAb (A6679)}

Immunohistochemistry - Osteoglycin (OGN) Rabbit pAb (A6679)

Immunohistochemistry analysis of paraffin-embedded Human stomach using Osteoglycin (OGN) Rabbit pAb (A6679) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Osteoglycin (OGN) Rabbit pAb (A6679)}

Immunofluorescence - Osteoglycin (OGN) Rabbit pAb (A6679)

Immunofluorescence analysis of U-2 OS cells using Osteoglycin (OGN) Rabbit pAb (A6679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)