제품 > 항체 > 다중클론항체(pAb)

Osteopontin Rabbit pAb (A1499)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunofluorescence analysis of Hep G2 cells using Osteopontin Rabbit pAb (A1499) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Osteopontin Rabbit pAb
Catalog No.	A1499
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 106-205 of human Osteopontin (NP_001035147.1).
Sequence	NDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAI
Gene ID
Swiss Prot
Synonyms	OPN; BNSP; BSPI; ETA-1; Osteopontin
Calculated MW	35kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Rat kidney
Cellular location	Secreted.
Customer validation	IHC(Homo sapiens, Rattus norvegicus, Mus musculus) WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus) ELISA(Homo sapiens, Mus musculus) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Osteopontin Rabbit pAb images

Immunofluorescence - Osteopontin Rabbit pAb (A1499)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Journal Of Cellular And Molecular Medicine

Application:

IHC

IF:

5.31

Species:

Homo sapiens

PMID:

33215860

Title:

Comprehensive analysis of partial epithelial mesenchymal transition‐related genes in hepatocellular carcinoma

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Macromol Biosci

Application:

IF:

4.97

Species:

Homo sapiens

PMID:

33713529

Title:

Fluoropolymer-Mediated Intracellular Delivery of miR-23b for the Osteocyte Differentiation in Osteoblasts

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Mol Med Rep

Application:

IHC

IF:

2.95

Species:

Rattus norvegicus

PMID:

34328202

Title:

Urantide prevents CCl4‑induced acute liver injury in rats by regulating the MAPK signalling pathway

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Acta Biochim Biophys Sin (Shanghai)

Application:

IF:

3.848

Species:

Homo sapiens

PMID:

34519779

Title:

Betulinic acid promotes the osteogenic differentiation of human periodontal ligament stem cells by upregulating EGR1

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

34707493

Title:

Screening and Identification of Hub Genes in the Corticosteroid Resistance Network in Human Airway Epithelial Cells via Microarray Analysis

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

36182943

Title:

Deubiquitylase OTUD1 confers Erlotinib sensitivity in non-small cell lung cancer through inhibition of nuclear translocation of YAP1 - PMC

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular cell research

Application:

IF:

5.01

Species:

Mus musculus

PMID:

37468072

Title:

CARM1 deficiency inhibits osteoblastic differentiation of bone marrow mesenchymal stem cells and delays osteogenesis in mice

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

ACS biomaterials science & engineering

Application:

IF:

5.39

Species:

Mus musculus

PMID:

37606611

Title:

Robust Osteoconductive -Tricalcium Phosphate/L-poly(lactic acid) Membrane via Orientation-Strengthening Technology

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IHC

IF:

11.092

Species:

Homo sapiens

PMID:

33694330

Title:

Biomimetic Silk Fibroin Hydrogels Strengthened by Silica Nanoparticles Distributed Nanofibers Facilitate Bone Repair

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

ELISA

IF:

6.081

Species:

Homo sapiens, Mus musculus

PMID:

35419364

Title:

Chaperone-Mediated Autophagy Ablation in Pericytes Reveals New Glioblastoma Prognostic Markers and Efficient Treatment Against Tumor Progression

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Frontiers in medicine

Application:

IHC

IF:

5.058

Species:

Homo sapiens, Mus musculus

PMID:

35514751

Title:

SPP1 and CXCL9 Promote Non-alcoholic Steatohepatitis Progression Based on Bioinformatics Analysis and Experimental Studies

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Eur J Pharmacol

Application:

WB, IHC

IF:

5.195

Species:

Rattus norvegicus

PMID:

33891969

Title:

Urantide alleviates the symptoms of atherosclerotic rats in vivo and in vitro models through the JAK2/STAT3 signaling pathway

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Rattus norvegicus

PMID:

35687823

Title:

Rosmarinic Acid Inhibits Platelet Aggregation and Neointimal Hyperplasia In Vivo and Vascular Smooth Muscle Cell Dedifferentiation, Proliferation, and Migration In

References for Osteopontin Rabbit pAb (A1499)

Product:

Osteopontin Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

38631227