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P23/PTGES3 Rabbit pAb (A5325)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - P23/PTGES3 Rabbit pAb (A5325)

Western blot analysis of various lysates using P23/PTGES3 Rabbit pAb (A5325) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameP23/PTGES3 Rabbit pAb
Catalog No.A5325
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human P23/P23/PTGES3 (NP_006592.3).
SequenceMQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTDRSILCCLRKGESGQSWPRLTKERAKLNWLS
Gene ID
Swiss Prot
SynonymsP23; TEBP; cPGES; P23/PTGES3
Calculated MW19kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Jurkat, Mouse testis, Rat testis
Cellular locationCytoplasm

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    ABclonal:Western blot - P23/PTGES3 Rabbit pAb (A5325)}

    Western blot - P23/PTGES3 Rabbit pAb (A5325)

    Western blot analysis of various lysates using P23/PTGES3 Rabbit pAb (A5325) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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