제품 > 항체 > 다중클론항체(pAb)

P2RX5 Rabbit pAb (A11710)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - P2RX5 Rabbit pAb (A11710)

Western blot analysis of various lysates using P2RX5 Rabbit pAb (A11710) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - P2RX5 Rabbit pAb (A11710)

Immunofluorescence analysis of Jurkat cells using P2RX5 Rabbit pAb (A11710) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - P2RX5 Rabbit pAb (A11710)

Immunofluorescence analysis of paraffin-embedded mouse spleen using P2RX5 Rabbit pAb (A11710) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameP2RX5 Rabbit pAb
Catalog No.A11710
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 341-422 of human P2RX5 (NP_002552.2).
SequenceKKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST
Gene ID
Swiss Prot
SynonymsP2X5; LRH-1; P2X5R; P2RX5
Calculated MW47kDa
Observed MW115kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNCI-H460, U-87MG, BxPC-3, K-562, Raji, Mouse heart, Mouse kidney, Mouse skeletal muscle, Rat heart
Cellular locationMembrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - P2RX5 Rabbit pAb (A11710)}

    Western blot - P2RX5 Rabbit pAb (A11710)

    Western blot analysis of various lysates using P2RX5 Rabbit pAb (A11710) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - P2RX5 Rabbit pAb (A11710)}

    Immunofluorescence - P2RX5 Rabbit pAb (A11710)

    Immunofluorescence analysis of Jurkat cells using P2RX5 Rabbit pAb (A11710) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - P2RX5 Rabbit pAb (A11710)}

    Immunofluorescence - P2RX5 Rabbit pAb (A11710)

    Immunofluorescence analysis of paraffin-embedded mouse spleen using P2RX5 Rabbit pAb (A11710) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.