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PABPN1 Rabbit mAb (A1735)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PABPN1 Rabbit mAb (A1735)

Western blot analysis of various lysates using PABPN1 Rabbit mAb (A1735) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of U-2 OS cells using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of paraffin-embedded Human colon cancer using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of C6 cells using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of C2C12 cells using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product namePABPN1 Rabbit mAb
Catalog No.A1735
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0730
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (Q86U42).
SequenceMAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGLESEELEPEELLLEPEPEPEPEEEPPRPRAPPGAPGPGPGSGAPGSQEEEE
Gene ID
Swiss Prot
SynonymsOPMD; PAB2; PABII; PABP2; PABP-2; PABPN1
Calculated MW31kDa/33kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, Raji, Mouse lung, Mouse brain, Rat brain
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PABPN1 Rabbit mAb (A1735)}

Western blot - PABPN1 Rabbit mAb (A1735)

Western blot analysis of various lysates using PABPN1 Rabbit mAb (A1735) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PABPN1 Rabbit mAb (A1735)}

Immunohistochemistry - PABPN1 Rabbit mAb (A1735)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using PABPN1 Rabbit mAb (A1735) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)}

Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of U-2 OS cells using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)}

Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of paraffin-embedded Human colon cancer using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)}

Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of C6 cells using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - PABPN1 Rabbit mAb (A1735)}

Immunofluorescence - PABPN1 Rabbit mAb (A1735)

Confocal imaging of C2C12 cells using PABPN1 Rabbit mAb (A1735, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1735? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)