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PAK1 Rabbit pAb (A3279)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PAK1 Rabbit pAb (A3279)

Western blot analysis of lysates from mouse kidney, using PAK1 Rabbit pAb (A3279) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - PAK1 Rabbit pAb (A3279)

Immunohistochemistry analysis of paraffin-embedded rat spleen using PAK1 Rabbit pAb (A3279) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PAK1 Rabbit pAb (A3279)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using PAK1 Rabbit pAb (A3279) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePAK1 Rabbit pAb
Catalog No.A3279
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human PAK1 (Q13153).
SequenceVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQ
Gene ID
Swiss Prot
SynonymsIDDMSSD; p65-PAK; PAKalpha; alpha-PAK; PAK1
Calculated MW61kDa
Observed MW82kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:25 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse kidney
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasm, focal adhesion, invadopodium, ruffle membrane
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - PAK1 Rabbit pAb (A3279)}

Western blot - PAK1 Rabbit pAb (A3279)

Western blot analysis of lysates from mouse kidney, using PAK1 Rabbit pAb (A3279) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - PAK1 Rabbit pAb (A3279)}

Immunohistochemistry - PAK1 Rabbit pAb (A3279)

Immunohistochemistry analysis of paraffin-embedded rat spleen using PAK1 Rabbit pAb (A3279) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PAK1 Rabbit pAb (A3279)}

Immunohistochemistry - PAK1 Rabbit pAb (A3279)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using PAK1 Rabbit pAb (A3279) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3279? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)