제품 > 항체 > 다중클론항체(pAb)

PALD1 Rabbit pAb (A12893)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PALD1 Rabbit pAb (A12893)

Western blot analysis of various lysates using PALD1 Rabbit pAb (A12893) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - PALD1 Rabbit pAb (A12893)

Immunofluorescence analysis of L929 cells using PALD1 Rabbit pAb (A12893) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePALD1 Rabbit pAb
Catalog No.A12893
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein tyrosine phosphatase activity. Predicted to be involved in peptidyl-tyrosine dephosphorylation. Located in cytosol.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PALD1 (NP_055246.2).
SequenceMGTTASTAQQTVSAGTPFEGLQGSGTMDSRHSVSIHSFQSTSLHNSKAKSIIPNKVAPVVITYNCKEEFQIHDELLKAHYTLGRLSDNTPEHYLVQGRYFLVRDVTEKMDVLGTVGSCGAPNFRQVQGGLTVFGMGQPSLSGFRRVLQKLQKDGHRECVIFCVREEPVLFLRADEDFVSYTPRDKQNLHENLQGLGPGVRVESLELAIRKEIHDFAQLSENTYHVYHNTEDLWGEPHAVAIHGEDDLHVTEEVYKRPLFLQPTYRYHRLPLPEQGSPLEAQLDAFVSVLRETPSLLQLRD
Gene ID
Swiss Prot
SynonymsPALD; KIAA1274; PALD1
Calculated MW97kDa
Observed MW97kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Mouse lung, Mouse heart, Mouse liver, Rat kidney
Cellular locationCytoplasm, cytosol

Documents

Certificate of Compliance

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    ABclonal:Western blot - PALD1 Rabbit pAb (A12893)}

    Western blot - PALD1 Rabbit pAb (A12893)

    Western blot analysis of various lysates using PALD1 Rabbit pAb (A12893) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - PALD1 Rabbit pAb (A12893)}

    Immunofluorescence - PALD1 Rabbit pAb (A12893)

    Immunofluorescence analysis of L929 cells using PALD1 Rabbit pAb (A12893) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.