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PARP8 Rabbit pAb (A12894)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PARP8 Rabbit pAb (A12894)

Western blot analysis of various lysates using PARP8 Rabbit pAb (A12894) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PARP8 Rabbit pAb (A12894)

Immunohistochemistry analysis of paraffin-embedded Human placenta using PARP8 Rabbit pAb (A12894) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePARP8 Rabbit pAb
Catalog No.A12894
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables protein ADP-ribosylase activity. Involved in protein auto-ADP-ribosylation and protein mono-ADP-ribosylation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP8 (NP_001171527.1).
SequenceMGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPRSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRIATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLSAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKS
Gene ID
Swiss Prot
SynonymsARTD16; pART16; PARP8
Calculated MW96kDa
Observed MW96kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse skeletal muscle, Rat skeletal muscle
Cellular location

Documents

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    ABclonal:Western blot - PARP8 Rabbit pAb (A12894)}

    Western blot - PARP8 Rabbit pAb (A12894)

    Western blot analysis of various lysates using PARP8 Rabbit pAb (A12894) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - PARP8 Rabbit pAb (A12894)}

    Immunohistochemistry - PARP8 Rabbit pAb (A12894)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using PARP8 Rabbit pAb (A12894) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.