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PASK Rabbit pAb (A8995)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PASK Rabbit pAb (A8995)

Western blot analysis of various lysates using PASK Rabbit pAb (A8995) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PASK Rabbit pAb (A8995)

Immunohistochemistry analysis of paraffin-embedded Human placenta using PASK Rabbit pAb (A8995) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PASK Rabbit pAb (A8995)

Immunohistochemistry analysis of paraffin-embedded Rat testis using PASK Rabbit pAb (A8995) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PASK Rabbit pAb (A8995)

Immunofluorescence analysis of C6 cells using PASK Rabbit pAb (A8995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PASK Rabbit pAb (A8995)

Immunofluorescence analysis of L929 cells using PASK Rabbit pAb (A8995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePASK Rabbit pAb
Catalog No.A8995
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1194-1323 of human PASK (NP_055963.2).
SequenceYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS
Gene ID
Swiss Prot
SynonymsSTK37; PASKIN; PASK
Calculated MW143kDa
Observed MW190kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, 22Rv1, Raji, Jurkat
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PASK Rabbit pAb (A8995)}

    Western blot - PASK Rabbit pAb (A8995)

    Western blot analysis of various lysates using PASK Rabbit pAb (A8995) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - PASK Rabbit pAb (A8995)}

    Immunohistochemistry - PASK Rabbit pAb (A8995)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using PASK Rabbit pAb (A8995) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - PASK Rabbit pAb (A8995)}

    Immunohistochemistry - PASK Rabbit pAb (A8995)

    Immunohistochemistry analysis of paraffin-embedded Rat testis using PASK Rabbit pAb (A8995) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - PASK Rabbit pAb (A8995)}

    Immunofluorescence - PASK Rabbit pAb (A8995)

    Immunofluorescence analysis of C6 cells using PASK Rabbit pAb (A8995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - PASK Rabbit pAb (A8995)}

    Immunofluorescence - PASK Rabbit pAb (A8995)

    Immunofluorescence analysis of L929 cells using PASK Rabbit pAb (A8995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)