제품 > 항체 > 단일클론항체(mAb)

PD-1/CD279 Mouse mAb (A20217)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Immunohistochemistry analysis of paraffin-embedded Human T-cell malignant lymphoma using PD-1/CD279 Mouse mAb (A20217) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using PD-1/CD279 Mouse mAb (A20217) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	PD-1/CD279 Mouse mAb
Catalog No.	A20217
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1, Kappa
CloneNo.	AMC0439

Background

Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 25-168 of human PD-1/CD279 (NP_005009.2).
Sequence	LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQT
Gene ID
Swiss Prot
Synonyms	PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD-1/CD279
Calculated MW	32kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃ Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerolpH7.3.
Key application	Immunohistochemistry
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein.
Customer validation	IF(Homo sapiens)