제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human PD-1/CD279 mAb (A25730)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293T cells (negative control, left) and MOLT-4 cells (right) were surface-stained with PE Rabbit anti-Human PD-1/CD279 mAb (A25730, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human PD-1/CD279 mAb (A25730, 5 μl/Test, right).

Flow cytometry: 1X10^6 Human PBMC (negative control, left) and Human PBMC (treated with PHA, right) were surface-stained with PE Rabbit anti-Human PD-1/CD279 mAb (A25730, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC (treated with PHA, right) were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human PD-1/CD279 mAb (A25730, 5 μl/Test, right).

Overview

Product name	PE Rabbit anti-Human PD-1/CD279 mAb
Catalog No.	A25730
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64145-PE

Background

Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 25-168 of human PD-1/CD279 (NP_005009.2).
Sequence	LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQT
Gene ID
Swiss Prot
Synonyms	PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Calculated MW	32kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Membrane, external side of plasma membrane, plasma membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Research Area

PE Rabbit anti-Human PD-1/CD279 mAb images

Flow CytoMetry - PE Rabbit anti-Human PD-1/CD279 mAb (A25730)

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human PD-1/CD279 mAb (A25730, 5 μl/Test, right).

Flow CytoMetry - PE Rabbit anti-Human PD-1/CD279 mAb (A25730)

* For research use only. Not for therapeutic or diagnostic purposes.