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PDAP1 Rabbit pAb (A4505)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDAP1 Rabbit pAb (A4505)

Western blot analysis of various lysates using PDAP1 Rabbit pAb (A4505) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PDAP1 Rabbit pAb (A4505)

Western blot analysis of lysates from Rat testis using PDAP1 Rabbit pAb (A4505) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - PDAP1 Rabbit pAb (A4505)

Immunofluorescence analysis of NIH/3T3 cells using PDAP1 Rabbit pAb (A4505) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePDAP1 Rabbit pAb
Catalog No.A4505
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a phosphoprotein that may upregulate the PDGFA-stimulated growth of fibroblasts and also downregulate the mitogenicity of PDGFB. The encoded protein in rodents has been shown to bind PDGFA with a low affinity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PDAP1 (NP_055706.1).
SequenceMPKGGRKGGHKGRARQYTSPEEIDAQLQAEKQKAREEEEQKEGGDGAAGDPKKEKKSLDSDESEDEEDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLD
Gene ID
Swiss Prot
SynonymsPAP; PAP1; HASPP28; PDAP1
Calculated MW21kDa
Observed MW26kDa/25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, HeLa, NIH/3T3, Rat testis, Rat testis, MCF7, HeLa, NIH/3T3
Cellular locationcytosol, extracellular region, plasma membrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PDAP1 Rabbit pAb (A4505)}

    Western blot - PDAP1 Rabbit pAb (A4505)

    Western blot analysis of various lysates using PDAP1 Rabbit pAb (A4505) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - PDAP1 Rabbit pAb (A4505)}

    Western blot - PDAP1 Rabbit pAb (A4505)

    Western blot analysis of lysates from Rat testis using PDAP1 Rabbit pAb (A4505) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - PDAP1 Rabbit pAb (A4505)}

    Immunofluorescence - PDAP1 Rabbit pAb (A4505)

    Immunofluorescence analysis of NIH/3T3 cells using PDAP1 Rabbit pAb (A4505) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.