제품 > 항체 > 다중클론항체(pAb)

PDCD10 Rabbit pAb (A17094)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDCD10 Rabbit pAb (A17094)

Western blot analysis of various lysates, using PDCD10 Rabbit pAb (A17094) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - PDCD10 Rabbit pAb (A17094)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using PDCD10 Rabbit pAb (A17094) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PDCD10 Rabbit pAb (A17094)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PDCD10 Rabbit pAb (A17094) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PDCD10 Rabbit pAb (A17094)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PDCD10 Rabbit pAb (A17094) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PDCD10 Rabbit pAb (A17094)

Immunofluorescence analysis of PC-12 cells using PDCD10 Rabbit pAb (A17094) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePDCD10 Rabbit pAb
Catalog No.A17094
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PDCD10 (NP_009148.2).
SequenceMRMTMEEMKNEAETTSMVSMPLYAVMYPVFNELERVNLSAAQTLRAAFIKAEKENPGLTQDIIMKILEKKSVEVNFTESLLRMAADDVEEYMIERPEPEFQDLNEKARALKQILSKIPDEINDRVRFLQTIKDIASAIKELLDTVNNVFKKYQYQNRRALEHQKKEFVKYSKSFSDTLKTYFKDGKAINVFVSANRLIHQTNLILQTFKTVA
Gene ID
Swiss Prot
SynonymsCCM3; TFAR15; PDCD10
Calculated MW25kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat lung, Rat spleen
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein.
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - PDCD10 Rabbit pAb (A17094)}

    Western blot - PDCD10 Rabbit pAb (A17094)

    Western blot analysis of various lysates, using PDCD10 Rabbit pAb (A17094) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - PDCD10 Rabbit pAb (A17094)}

    Immunohistochemistry - PDCD10 Rabbit pAb (A17094)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using PDCD10 Rabbit pAb (A17094) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - PDCD10 Rabbit pAb (A17094)}

    Immunohistochemistry - PDCD10 Rabbit pAb (A17094)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PDCD10 Rabbit pAb (A17094) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - PDCD10 Rabbit pAb (A17094)}

    Immunohistochemistry - PDCD10 Rabbit pAb (A17094)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PDCD10 Rabbit pAb (A17094) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - PDCD10 Rabbit pAb (A17094)}

    Immunofluorescence - PDCD10 Rabbit pAb (A17094)

    Immunofluorescence analysis of PC-12 cells using PDCD10 Rabbit pAb (A17094) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A17094? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)