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PDHA2 Rabbit pAb (A14994)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDHA2 Rabbit pAb (A14994)

Western blot analysis of various lysates using PDHA2 Rabbit pAb (A14994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A14994)

Immunofluorescence analysis of L929 cells using PDHA2 Rabbit pAb (A14994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePDHA2 Rabbit pAb
Catalog No.A14994
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable pyruvate dehydrogenase (acetyl-transferring) activity. Predicted to contribute to pyruvate dehydrogenase (NAD+) activity. Predicted to be involved in acetyl-CoA biosynthetic process from pyruvate. Predicted to act upstream of or within mitochondrial acetyl-CoA biosynthetic process from pyruvate. Located in mitochondrion. Is expressed in nervous system and testis. Orthologous to human PDHA2 (pyruvate dehydrogenase E1 subunit alpha 2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 230-391 of mouse PDHA2 (NP_032837.1).
SequenceMGTSNERSAASTDYHKKGFIIPGLRVNGMDILCVREATKFAADHCRSGKGPIVMELQTYRYHGHSMSDPGISYRSREEVHNVRSKSDPIMLLRERIISNNLSNIEELKEIDADVKKEVEDAAQFATTDPEPAVEDIANYLYHQDPPFEVRGAHKWLKYKSHS
Gene ID
Swiss Prot
SynonymsPdhal; PDHA2
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293T, Mouse brain, Mouse kidney, Rat brain
Cellular locationMitochondrion matrix

Documents

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    ABclonal:Western blot - PDHA2 Rabbit pAb (A14994)}

    Western blot - PDHA2 Rabbit pAb (A14994)

    Western blot analysis of various lysates using PDHA2 Rabbit pAb (A14994) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A14994)}

    Immunofluorescence - PDHA2 Rabbit pAb (A14994)

    Immunofluorescence analysis of L929 cells using PDHA2 Rabbit pAb (A14994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.