제품 > 항체 > 다중클론항체(pAb)

PDHA2 Rabbit pAb (A9943)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDHA2 Rabbit pAb (A9943)

Western blot analysis of various lysates using PDHA2 Rabbit pAb (A9943) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A9943)

Immunofluorescence analysis of C6 cells using PDHA2 Rabbit pAb (A9943) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A9943)

Immunofluorescence analysis of HeLa cells using PDHA2 Rabbit pAb (A9943) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A9943)

Immunofluorescence analysis of NIH-3T3 cells using PDHA2 Rabbit pAb (A9943) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePDHA2 Rabbit pAb
Catalog No.A9943
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables pyruvate dehydrogenase (acetyl-transferring) activity. Involved in pyruvate metabolic process. Located in mitochondrion and nucleolus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 119-388 of human PDHA2 (NP_005381.1).
SequenceHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS
Gene ID
Swiss Prot
SynonymsPDHAL; SPGF70; PDHA2
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNCI-H460, SKOV3, A-549, Mouse testis, Mouse ovary
Cellular locationMitochondrion matrix.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PDHA2 Rabbit pAb (A9943)}

Western blot - PDHA2 Rabbit pAb (A9943)

Western blot analysis of various lysates using PDHA2 Rabbit pAb (A9943) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A9943)}

Immunofluorescence - PDHA2 Rabbit pAb (A9943)

Immunofluorescence analysis of C6 cells using PDHA2 Rabbit pAb (A9943) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A9943)}

Immunofluorescence - PDHA2 Rabbit pAb (A9943)

Immunofluorescence analysis of HeLa cells using PDHA2 Rabbit pAb (A9943) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PDHA2 Rabbit pAb (A9943)}

Immunofluorescence - PDHA2 Rabbit pAb (A9943)

Immunofluorescence analysis of NIH-3T3 cells using PDHA2 Rabbit pAb (A9943) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)