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PDK2 Rabbit mAb (A4737)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDK2 Rabbit mAb (A4737)

Western blot analysis of various lysates using PDK2 Rabbit mAb (A4737) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - PDK2 Rabbit mAb (A4737)

Immunohistochemistry analysis of paraffin-embedded Human colon using PDK2 Rabbit mAb (A4737) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePDK2 Rabbit mAb
Catalog No.A4737
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1113
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 308-407 of human PDK2 (Q15119).
SequenceYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS
Gene ID
Swiss Prot
SynonymsPDHK2; PDKII; PDK2
Calculated MW39kDa/46kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Mouse heart, Rat heart
Cellular locationMitochondrion matrix
Customer validation

WB(Sus scrofa, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - PDK2 Rabbit mAb (A4737)}

Western blot - PDK2 Rabbit mAb (A4737)

Western blot analysis of various lysates using PDK2 Rabbit mAb (A4737) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - PDK2 Rabbit mAb (A4737)}

Immunohistochemistry - PDK2 Rabbit mAb (A4737)

Immunohistochemistry analysis of paraffin-embedded Human colon using PDK2 Rabbit mAb (A4737) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4737? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)