제품 > 항체 > 다중클론항체(pAb)

PDK2 Rabbit pAb (A4012)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDK2 Rabbit pAb (A4012)

Western blot analysis of extracts of various cell lines, using PDK2 antibody (A4012) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - PDK2 Rabbit pAb (A4012)

Western blot analysis of extracts of BxPC-3 cells, using PDK2 antibody (A4012) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - PDK2 Rabbit pAb (A4012)

Immunofluorescence analysis of U2OS cells using PDK2 antibody (A4012) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePDK2 Rabbit pAb
Catalog No.A4012
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 128-407 of human PDK2 (NP_002602.2).
SequencePTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS
Gene ID
Swiss Prot
SynonymsPDHK2; PDKII
Calculated MW46kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesBxPC-3, Mouse brain, Mouse heart, Rat heart
Cellular locationMitochondrion matrix
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PDK2 Rabbit pAb (A4012)}

Western blot - PDK2 Rabbit pAb (A4012)

Western blot analysis of extracts of various cell lines, using PDK2 antibody (A4012) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - PDK2 Rabbit pAb (A4012)}

Western blot - PDK2 Rabbit pAb (A4012)

Western blot analysis of extracts of BxPC-3 cells, using PDK2 antibody (A4012) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - PDK2 Rabbit pAb (A4012)}

Immunofluorescence - PDK2 Rabbit pAb (A4012)

Immunofluorescence analysis of U2OS cells using PDK2 antibody (A4012) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4012? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)