제품 > 항체 > 다중클론항체(pAb)

PDXDC1 Rabbit pAb (A14856)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDXDC1 Rabbit pAb (A14856)

Western blot analysis of various lysates using PDXDC1 Rabbit pAb (A14856) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PDXDC1 Rabbit pAb (A14856)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PDXDC1 Rabbit pAb (A14856) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePDXDC1 Rabbit pAb
Catalog No.A14856
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cadherin binding activity. Predicted to be involved in carboxylic acid metabolic process. Located in Golgi apparatus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 489-788 of human PDXDC1 (NP_055842.2).
SequenceLCCTLQLREEFKQEVEATAGLLYVDDPNWSGIGVVRYEHANDDKSSLKSDPEGENIHAGLLKKLNELESDLTFKIGPEYKSMKSCLYVGMASDNVDAAELVETIAATAREIEENSRLLENMTEVVRKGIQEAQVELQKASEERLLEEGVLRQIPVVGSVLNWFSPVQALQKGRTFNLTAGSLESTEPIYVYKAQGAGVTLPPTPSGSRTKQRLPGQKPFKRSLRGSDALSETSSVSHIEDLEKVERLSSGPEQITLEASSTEGHPGAPSPQHTDQTEAFQKGVPHPEDDHSQVEGPESLR
Gene ID
Swiss Prot
SynonymsLP8165; PDXDC1
Calculated MW87kDa
Observed MW87-106kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-251MG, A-549, HeLa, Mouse testis, Mouse brain, Rat heart
Cellular locationGolgi apparatus

Documents

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ABclonal:Western blot - PDXDC1 Rabbit pAb (A14856)}

Western blot - PDXDC1 Rabbit pAb (A14856)

Western blot analysis of various lysates using PDXDC1 Rabbit pAb (A14856) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - PDXDC1 Rabbit pAb (A14856)}

Immunohistochemistry - PDXDC1 Rabbit pAb (A14856)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PDXDC1 Rabbit pAb (A14856) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.