제품 > 항체 > 다중클론항체(pAb)

PELI1 Rabbit pAb (A8240)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - PELI1 Rabbit pAb (A8240)

Western blot analysis of extracts of rat brain, using PELI1 antibody (A8240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product namePELI1 Rabbit pAb
Catalog No.A8240
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ubiquitin protein ligase activity. Involved in several processes, including negative regulation of necroptotic process; protein polyubiquitination; and response to lipopolysaccharide. Predicted to be located in cytosol.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PELI1 (NP_065702.2).
SequenceMFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGAT
Gene ID
Swiss Prot
SynonymsPELI1
Calculated MW46kDa
Observed MW46kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat brain
Cellular locationcytosol, nucleus
ABclonal:Western blot - PELI1 Rabbit pAb (A8240)}

Western blot - PELI1 Rabbit pAb (A8240)

Western blot analysis of extracts of rat brain, using PELI1 antibody (A8240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)