제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human CD1B mAb (A26121)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and MOLT-4 cells (right) were surface-stained with PE Rabbit anti-Human CD1B mAb (A26121, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD45RB mAb (A26117, 5 μl/Test, right).

Overview

Product name	PE Rabbit anti-Human CD1B mAb
Catalog No.	A26121
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66962-PE

Background

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human CD1B (NP_001755.1).
Sequence	SEHAFQGPTSFHVIQTSSFTNSTWAQTQGSGWLDDLQIHGWDSDSGTAIFLKPWSKGNFSDKEVAELEEIFRVYIFGFAREVQDFAGDFQMKYPFEIQGIAGCELHSGGAIVSFLRGALGGLDFLSVKNASCVPSPEGGSRAQKFCALIIQYQGIMETVRILLYETCPRYLLGVLNAGKADLQRQVKPEAWLSSGPSPGPGRLQLVCHVSGFYPKPVWVMWMRGEQEQQGTQLGDILPNANWTWYLRATLDVADGEAAGLSCRVKHSSLEGQDIILYWRNPTSIGS
Gene ID
Swiss Prot
Synonyms	R1; CD1; CD1A
Calculated MW	37kDa/30kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

PE Rabbit anti-Human CD1B mAb images

Flow CytoMetry - PE Rabbit anti-Human CD1B mAb (A26121)

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD45RB mAb (A26117, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.