제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human HIF-1 alpha mAb (A27280)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Rabbit anti-Human HIF-1 alpha mAb (A27280)

Flow cytometry:1X10^6 HeLa cells (untreated, left) and HeLa cells (treated with Deferoxamine, right) were intracellularly-stained with PE Rabbit anti-Human HIF-1 alpha mAb (A27280, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human HIF-1 alpha mAb (A27280)

Flow cytometry: 1X10^6 HeLa cells (untreated, left) and HeLa cells (treated with Deferoxamine, right) were intracellularly-stained with PE Rabbit anti-Human HIF-1 alpha mAb (A27280, 5 μl/Test).

Overview

Product namePE Rabbit anti-Human HIF-1 alpha mAb
Catalog No.A27280
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC71404-PE
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 401-500 of HIF1 alpha (NP_001521.1).
SequenceAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPS
Gene ID
Swiss Prot
SynonymsHIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Calculated MW93kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationaxon cytoplasm, cytoplasm, cytosol, nuclear body, nuclear speck, nucleoplasm, nucleus, Cytoplasm, Nucleus, Nucleus speckle.

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ABclonal:Flow CytoMetry - PE Rabbit anti-Human HIF-1 alpha mAb (A27280)}

Flow CytoMetry - PE Rabbit anti-Human HIF-1 alpha mAb (A27280)

Flow cytometry:1X10^6 HeLa cells (untreated, left) and HeLa cells (treated with Deferoxamine, right) were intracellularly-stained with PE Rabbit anti-Human HIF-1 alpha mAb (A27280, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human HIF-1 alpha mAb (A27280)}

Flow CytoMetry - PE Rabbit anti-Human HIF-1 alpha mAb (A27280)

Flow cytometry: 1X10^6 HeLa cells (untreated, left) and HeLa cells (treated with Deferoxamine, right) were intracellularly-stained with PE Rabbit anti-Human HIF-1 alpha mAb (A27280, 5 μl/Test).

* For research use only. Not for therapeutic or diagnostic purposes.

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (25)