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PE Rabbit anti-Mouse CD103 mAb (A27132)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD103 mAb (A27132)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes cells (right) were surface-stained with PE Rabbit anti-Mouse CD103 mAb (A27132, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD103 mAb (A27132)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD103 mAb (A27132, 5 μl/Test, right).

Overview

Product namePE Rabbit anti-Mouse CD103 mAb
Catalog No.A27132
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC69162-PE
Predicted to enable metal ion binding activity. Predicted to act upstream of or within cell adhesion and integrin-mediated signaling pathway. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; genitourinary system; lymph node; nasal septum; and nucleus pulposus. Orthologous to human ITGAE (integrin subunit alpha E).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 191-385 of mouse CD103 (NP_032425.2).
SequenceEDGTEIAIVLDGSGSIEPSDFQKAKNFISTMMRNFYEKCFECNFALVQYGAVIQTEFDLQESRDINASLAKVQSIVQVKEVTKTASAMQHVLDNIFIPSRGSRKKALKVMVVLTDGDIFGDPLNLTTVINSPKMQGVVRFAIGVGDAFKNNNTYRELKLIASDPKEAHTFKVTNYSALDGLLSKLQQHIVHMEGT
Gene ID
Swiss Prot
SynonymsCD103; aM290; alpha-E1; A530055J10; alpha-M290
Calculated MW129kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD103 mAb (A27132)}

    Flow CytoMetry - PE Rabbit anti-Mouse CD103 mAb (A27132)

    Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes cells (right) were surface-stained with PE Rabbit anti-Mouse CD103 mAb (A27132, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - PE Rabbit anti-Mouse CD103 mAb (A27132)}

    Flow CytoMetry - PE Rabbit anti-Mouse CD103 mAb (A27132)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD103 mAb (A27132, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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