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PEX19 Rabbit pAb (A5476)

Reviews (2)Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PEX19 Rabbit pAb (A5476)

Western blot analysis of extracts of various cell lines, using PEX19 antibody (A5476) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PEX19 Rabbit pAb (A5476)

Immunofluorescence analysis of mouse liver using PEX19 antibody (A5476) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for PEX19 Rabbit pAb(A5476)
Review
ABclonal: review for PEX19 Rabbit pAb(A5476)

Overview

Product namePEX19 Rabbit pAb
Catalog No.A5476
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. These disorders have at least 14 complementation groups, with more than one phenotype being observed for some complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14), which is also known as PBD-CGJ. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human PEX19 (NP_002848.1).
SequenceMAAAEEGCSVGAEADRELEELLESALDDFDKAKPSPAPPSTTTAPDASGPQKRSPGDTAKDALFASQEKFFQELFDSELASQATAEFEKAMKELAEEEPHLVEQFQKLSEAAGRVGSDMTSQQEFTSCLKETLSGLAKNATDLQNSSMSEEELTKAMEGLGMDEGDGEGNILPIMQSIMQNLLSKDVLYPSLKEITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCKICEQFEAETPTDSETTQKARFEMVLDLMQQLQDLGHPPKELAGEMPPGLNFDLDALNLSGPPGASGEQCLIM
Gene ID
Swiss Prot
SynonymsPXF; HK33; PMP1; PMPI; PXMP1; PBD12A; D1S2223E
Calculated MW33kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples22Rv1, THP-1, U-87MG, Jurkat
Cellular locationCytoplasm, Cytoplasmic side, Lipid-anchor, Peroxisome membrane
Customer validation

(3T3-L1, HepG2)

WB(Mus musculus, Homo sapiens)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - PEX19 Rabbit pAb (A5476)}

    Western blot - PEX19 Rabbit pAb (A5476)

    Western blot analysis of extracts of various cell lines, using PEX19 antibody (A5476) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - PEX19 Rabbit pAb (A5476)}

    Immunofluorescence - PEX19 Rabbit pAb (A5476)

    Immunofluorescence analysis of mouse liver using PEX19 antibody (A5476) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (1)

    Secondary Antibodies (26)