제품 > 항체 > 다중클론항체(pAb)

PFN4 Rabbit pAb (A13692)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PFN4 Rabbit pAb (A13692)

Western blot analysis of various lysates using PFN4 Rabbit pAb (A13692) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

Overview

Product namePFN4 Rabbit pAb
Catalog No.A13692
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable actin monomer binding activity. Predicted to be involved in sequestering of actin monomers. Predicted to be located in cytoskeleton. Predicted to be active in cell cortex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-129 of human PFN4 (NP_955378.1).
SequenceMSHLQSLLLDTLLGTKHVDSAALIKIQERSLCVASPGFNVTPSDVRTLVNGFAKNPLQARREGLYFKGKDYRCVRADEYSLYAKNENTGVVVVKTHLYLLVATYTEGMYPSICVEATESLGDYLRKKGS
Gene ID
Swiss Prot
SynonymsPFN4
Calculated MW14kDa
Observed MW14kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse heart, Rat heart
Cellular locationCytoplasm, cytoskeleton
ABclonal:Western blot - PFN4 Rabbit pAb (A13692)}

Western blot - PFN4 Rabbit pAb (A13692)

Western blot analysis of various lysates using PFN4 Rabbit pAb (A13692) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

* For research use only. Not for therapeutic or diagnostic purposes.