제품 > 항체 > 단일클론항체(mAb)

PGK1 Rabbit mAb (A12686)

Review (1)Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PGK1 Rabbit mAb (A12686)

Western blot analysis of various lysates using PGK1 Rabbit mAb (A12686) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for PGK1 Rabbit mAb(A12686)

Overview

Product namePGK1 Rabbit mAb
Catalog No.A12686
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0700
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PGK1 (P00558).
SequenceMSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCV
Gene ID
Swiss Prot
SynonymsPGKA; MIG10; HEL-S-68p; PGK1
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HepG2, K-562, Mouse lung, Mouse brain, Rat ovary, Rat kidney
Cellular locationCytoplasm.
Customer validation

(MCL-4、HL-60、PC-3)

WB(Mus musculus, Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PGK1 Rabbit mAb (A12686)}

Western blot - PGK1 Rabbit mAb (A12686)

Western blot analysis of various lysates using PGK1 Rabbit mAb (A12686) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A12686

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A12686? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

재조합 단백질 (1)

Secondary Antibodies (26)