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PGK1 Rabbit pAb (A14039)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PGK1 Rabbit pAb (A14039)

Western blot analysis of various lysates, using PGK1 Rabbit pAb (A14039) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded rat intestine using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded human colon using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded human esophagus using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product namePGK1 Rabbit pAb
Catalog No.A14039
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-417 of human PGK1 (NP_000282.1).
SequenceMSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
Gene ID
Swiss Prot
SynonymsPGKA; MIG10; HEL-S-68p; PGK1
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-251MG, HepG2, HeLa, Mouse brain
Cellular locationCytoplasm
Customer validation

WB(Aspergillus flavus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - PGK1 Rabbit pAb (A14039)}

Western blot - PGK1 Rabbit pAb (A14039)

Western blot analysis of various lysates, using PGK1 Rabbit pAb (A14039) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)}

Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded rat intestine using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)}

Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded human colon using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)}

Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded human esophagus using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PGK1 Rabbit pAb (A14039)}

Immunohistochemistry - PGK1 Rabbit pAb (A14039)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PGK1 Rabbit pAb (A14039) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14039? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

재조합 단백질 (1)

Secondary Antibodies (26)