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PHD3 Rabbit mAb (A21225)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PHD3 Rabbit mAb (A21225)

Western blot analysis of various lysates using PHD3 Rabbit mAb (A21225) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - PHD3 Rabbit mAb (A21225)

Western blot analysis of lysates from BxPC-3 cells, using PHD3 Rabbit mAb (A21225) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Human placenta using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - PHD3 Rabbit mAb (A21225)

Confocal imaging of U-2 OS cells using PHD3 Rabbit mAb (A21225, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product namePHD3 Rabbit mAb
Catalog No.A21225
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52452
Enables peptidyl-proline 4-dioxygenase activity. Involved in several processes, including activation of cysteine-type endopeptidase activity involved in apoptotic process; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; and response to hypoxia. Located in cytosol and nucleus. Implicated in renal cell carcinoma. Biomarker of clear cell renal cell carcinoma.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD3 (NP_071356.1).
SequenceMPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHCTGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAISFLLSLIDR
Gene ID
Swiss Prot
SynonymsPHD3; HIFPH3; HIFP4H3
Calculated MW27kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 40000
  • IHC-P 1:100 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBxPC-3, Mouse kidney, Mouse brain
Cellular locationCytoplasm, Nucleus.
Customer validation

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - PHD3 Rabbit mAb (A21225)}

Western blot - PHD3 Rabbit mAb (A21225)

Western blot analysis of various lysates using PHD3 Rabbit mAb (A21225) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - PHD3 Rabbit mAb (A21225)}

Western blot - PHD3 Rabbit mAb (A21225)

Western blot analysis of lysates from BxPC-3 cells, using PHD3 Rabbit mAb (A21225) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)}

Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)}

Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)}

Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Human placenta using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PHD3 Rabbit mAb (A21225)}

Immunohistochemistry - PHD3 Rabbit mAb (A21225)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using PHD3 Rabbit mAb (A21225) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - PHD3 Rabbit mAb (A21225)}

Immunofluorescence - PHD3 Rabbit mAb (A21225)

Confocal imaging of U-2 OS cells using PHD3 Rabbit mAb (A21225, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A21225? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)