Western blot - PHGDH Rabbit mAb (A22129)
Western blot analysis of various lysates using PHGDH Rabbit mAb (A22129) at1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):MCF7.
Exposure time: 30s.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - PHGDH Rabbit mAb (A22129)
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using PHGDH Rabbit mAb (A22129) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - PHGDH Rabbit mAb (A22129)
Confocal imaging of HeLa cells using PHGDH Rabbit mAb (A22129, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - PHGDH Rabbit mAb (A22129)
Confocal imaging of NIH/3T3 cells using PHGDH Rabbit mAb (A22129, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.