제품 > 항체 > 다중클론항체(pAb)

PI3KP85α/P55γ/P85β Rabbit pAb (A18355)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PIK3R1/PIK3R2/PIK3R3 Rabbit pAb (A18355) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	PI3KP85α/P55γ/P85β Rabbit pAb
Catalog No.	A18355
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 400 to 500 of human PIK3R1/PIK3R2/PIK3R3 (NP_852664.1).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	PIK3R1/PIK3R2/PIK3R3; PI3KP85α/P55γ/P85β
Calculated MW	42-54kDa/81-84kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jukat, NIH/3T3, Mouse brain, Rat brain, Rat liver
Cellular location