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PIN4 Rabbit mAb (A20919)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PIN4 Rabbit mAb (A20919)

Western blot analysis of extracts of various cell lines, using PIN4 antibody (A20919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePIN4 Rabbit mAb
Catalog No.A20919
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2893
This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 52-131 of human PIN4 (Q9Y237).
SequenceMEAMEKLKSGMRFNEVAAQYSEDKARQGGDLGWMTRGSMVGPFQEAAFALPVSGMDKPVFTDPPVKTKFGYHIIMVEGRK
Gene ID
Swiss Prot
SynonymsEPVH; PAR14; PAR17; hEPVH; hPar14; hPar17
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, HepG2, U-251MG, Mouse liver, Rat brain, Rat thymus
Cellular locationcytoplasm, mitochondrial matrix, nucleolus, nucleoplasm, nucleus, spindle

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ABclonal:Western blot - PIN4 Rabbit mAb (A20919)}

Western blot - PIN4 Rabbit mAb (A20919)

Western blot analysis of extracts of various cell lines, using PIN4 antibody (A20919) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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