제품 > 항체 > 다중클론항체(pAb)

PITPNA Rabbit pAb (A12966)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PITPNA Rabbit pAb (A12966)

Western blot analysis of various lysates using PITPNA Rabbit pAb (A12966) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - PITPNA Rabbit pAb (A12966)

Immunofluorescence analysis of L929 cells using PITPNA Rabbit pAb (A12966) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePITPNA Rabbit pAb
Catalog No.A12966
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) by phosphoinositide-3-kinase.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 211-270 of human PITPNA (NP_006215.1).
SequenceNFIHKQERRLFTNFHRQLFCWLDKWVDLTMDDIRRMEEETKRQLDEMRQKDPVKGMTADD
Gene ID
Swiss Prot
SynonymsPITPN; VIB1A; HEL-S-36; PI-TPalpha; PITPNA
Calculated MW32kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart
Cellular locationCytoplasm

Documents

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    ABclonal:Western blot - PITPNA Rabbit pAb (A12966)}

    Western blot - PITPNA Rabbit pAb (A12966)

    Western blot analysis of various lysates using PITPNA Rabbit pAb (A12966) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - PITPNA Rabbit pAb (A12966)}

    Immunofluorescence - PITPNA Rabbit pAb (A12966)

    Immunofluorescence analysis of L929 cells using PITPNA Rabbit pAb (A12966) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.