제품 > 항체 > 다중클론항체(pAb)

PITPNM1 Rabbit pAb (A13063)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PITPNM1 Rabbit pAb (A13063) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	PITPNM1 Rabbit pAb
Catalog No.	A13063
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

PITPNM1 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al., 2005 [PubMed 15627748]).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 110-190 of human PITPNM1 (NP_004901.2).
Sequence	EIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAY
Gene ID
Swiss Prot
Synonyms	Rd9; NIR2; RDGB; DRES9; RDGB1; RDGBA; PITPNM; RDGBA1; PITPNM1
Calculated MW	135kDa
Observed MW	135kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	LO2, Mouse brain, Mouse eye, Rat brain
Cellular location	Cleavage furrow, Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus, Golgi stack membrane, Lipid droplet, Midbody, Peripheral membrane protein