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PIWIL1 Rabbit mAb (A3490)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PIWIL1 Rabbit mAb (A3490)

Western blot analysis of various lysates using PIWIL1 Rabbit mAb (A3490) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - PIWIL1 Rabbit mAb (A3490)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PIWIL1 Rabbit mAb (A3490) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PIWIL1 Rabbit mAb (A3490)

Confocal imaging of paraffin-embedded Rat testis using PIWIL1 Rabbit mAb (A3490, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - PIWIL1 Rabbit mAb (A3490)

Confocal imaging of paraffin-embedded Mouse testis using PIWIL1 Rabbit mAb (A3490, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product namePIWIL1 Rabbit mAb
Catalog No.A3490
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0786
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-800 of human PIWIL1 (Q96J94).
SequenceYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN
Gene ID
Swiss Prot
SynonymsHIWI; MIWI; PIWI; CT80.1; PIWIL1
Calculated MW99kDa
Observed MW90kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse testis, Mouse brain, Rat testis, Rat brain
Cellular locationCytoplasm
Customer validation

IF(Mus musculus)

WB(Mus musculus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - PIWIL1 Rabbit mAb (A3490)}

    Western blot - PIWIL1 Rabbit mAb (A3490)

    Western blot analysis of various lysates using PIWIL1 Rabbit mAb (A3490) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - PIWIL1 Rabbit mAb (A3490)}

    Immunohistochemistry - PIWIL1 Rabbit mAb (A3490)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using PIWIL1 Rabbit mAb (A3490) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - PIWIL1 Rabbit mAb (A3490)}

    Immunofluorescence - PIWIL1 Rabbit mAb (A3490)

    Confocal imaging of paraffin-embedded Rat testis using PIWIL1 Rabbit mAb (A3490, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - PIWIL1 Rabbit mAb (A3490)}

    Immunofluorescence - PIWIL1 Rabbit mAb (A3490)

    Confocal imaging of paraffin-embedded Mouse testis using PIWIL1 Rabbit mAb (A3490, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A3490? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)