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PKC-beta Rabbit mAb (A21241)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PKC-beta Rabbit mAb (A21241)

Western blot analysis of various lysates using PKC-beta Rabbit mAb (A21241) at1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PKC-beta Rabbit mAb (A21241)

Western blot analysis of various lysates using PKC-beta Rabbit mAb (A21241) at1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded mouse spleen tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded rat brain tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded rat spleen tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded mouse brain tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded human colon tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product namePKC-beta Rabbit mAb
Catalog No.A21241
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52364
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 260-340 of human PKC-beta (NP_997700.1).
SequenceSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLT
Gene ID
Swiss Prot
SynonymsPKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
Calculated MW77kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4800
  • IHC-P 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, SK-MEL-28, Mouse brain, Rat brain
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein
Customer validation

WB(Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PKC-beta Rabbit mAb (A21241)}

Western blot - PKC-beta Rabbit mAb (A21241)

Western blot analysis of various lysates using PKC-beta Rabbit mAb (A21241) at1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - PKC-beta Rabbit mAb (A21241)}

Western blot - PKC-beta Rabbit mAb (A21241)

Western blot analysis of various lysates using PKC-beta Rabbit mAb (A21241) at1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)}

Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded mouse spleen tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)}

Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded rat brain tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)}

Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded rat spleen tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)}

Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded mouse brain tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PKC-beta Rabbit mAb (A21241)}

Immunohistochemistry - PKC-beta Rabbit mAb (A21241)

Immunohistochemistry analysis of PKC-beta in paraffin-embedded human colon tissue using PKC-beta Rabbit mAb (A21241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)